A woman is on a life support machine and a three-year-old girl is seriously ill after an E.coli outbreak, with a possible link to a fish and chip shop.
A council inspection of a chip shop possibly linked to an E.Coli outbreak found poor hygiene conditions and major non-compliance with food legislation.
The family of Karen Morrisroe-Clutton, from Wrexham, who has an 11-week-old baby, said she fell ill after eating a vegetarian burger.
Wrexham Council, which has been asked to comment, gave the Llay Fish Bar its lowest possible star rating - zero stars- in an inspection a year ago.
A three-year-old girl has renal failure in a Liverpool hospital.
Karen Morrisroe-Clutton, 32, and a three-year-old girl remain "seriously ill" in hospital with E.coli.
Four cases are being investigated. The chip shop has been shut but the owner said he used respectable suppliers.
The chip shop owner's solicitor said he was cooperating fully with the inquiry.
Llay Fish Bar, Llay, Wrexham has been closed since 30 July and an indefinite closure order was granted by the town's magistrates on Monday.
According to Wrexham council's website, Llay Fish Bar was inspected on 13 August, 2008.
A Wrexham council spokeswoman said: "It will remain closed until we're satisfied that health requirements and legislation have been met."
The council noted: "Poor hygiene conditions found. Some major non-compliance with food legislation."
Karen Morrisroe-Clutton with baby son Oliver She's still very critical but the doctors, who are absolutely out of this world, remain optimistic Paul Clutton, husband
The chip shop was recommended for annual inspections, and the next is due this month.
The three-year-old girl is being treated at Alder Hey Hospital, Liverpool.
Ms Morrisroe-Clutton, who is on a life support system, has an 11-week-old baby son.
Karen Morrisroe-Clutton, a librarian, is being treated at Wrexham Maelor Hospital, where her husband Paul has been at her bedside.
Her husband, Paul Clutton, said: "She's got a lot to fight for - little baby Oliver wants his mum back."
The hospital said this evening she was in a "seriously ill" condition.
Ms Morrisroe-Clutton's son, Oliver, is being cared for by her parents
Mr Clutton earlier told BBC Wales: "She's still very critical but the doctors, who are absolutely out of this world, remain optimistic, but at any moment it could get worse.
Four cases of E.coli are being investigated by Wales' public health chiefs and the local authority's environmental health officers.
"She's stable and her body is fighting this disease."
The chip shop, Llay Fish Bar, in the Llay area of the town, has been shut but the owner, who has 17 years' experience, said he used respectable suppliers.
Ms Morrisroe-Clutton's mother, Rose Morrisroe, who is looking after her daughter's baby Oliver, said she fell ill last Tuesday after eating a vegetarian burger from the chip shop.
The family of Ms Morrisroe-Clutton said she is being treated in a medically-induced coma in Wrexham Maelor Hospital since falling ill last Tuesday after eating a vegetarian burger. Her condition has not changed overnight.
"The doctor became worried and sent for tests, after which she was admitted to intensive care at the Maelor Hospital.
The three-year-old girl is in Alder Hey Hospital in Liverpool with renal failure. The hospital said her condition remains stable.
"She's been in a medically-induced coma since then and is on kidney dialysis and a plasma exchange machine.
The leader of Wrexham council, Aled Roberts, said the council had last week completed its investigations under the Pennington report, the public inquiry into the E.coli outbreak in 2005 which left one boy dead and over 150 other people ill.
Public health consultant Dr Judy Hart said they looked at different possible sources
He said the council had checked "high risk" establishments, and Llay Fish Bar was not among them.
"Things are improving slightly but she's not out of danger yet. She's just had a baby which we're looking after.
He said: "We'd concentrated on meat establishments. Fish and chip shops generally are considered to be low risk.
"Thankfully he's young enough not to know. It's a very worrying time."
"My understanding in the briefing last Friday was that the Llay establishment was due for an annual check on 14 August.
Pierre Bartlett, the solicitor for Ramazan Aslan, owner of the fish bar, said his client was co-operating fully with the investigation. His thoughts were with the families of those who are ill.
'Thoughts'
Mr Bartlett said Mr Aslan runs a clean and sensible establishment and uses respectable food suppliers.
"It's national standards for there to be one [annual] check in low-risk establishments.
An environmental health officer told Wrexham magistrates that two of the four cases tested had been confirmed as being a common strain.
"As far as I was concerned, it was clear because if there had been any problems, then action would have been taken but I think it's important to note that at the moment it's not confirmed that this particular establishment is the source."
Professor Hugh Pennington says the latest outbreak in Wrexham could have occured at the food preparation stage
Pierre Bartlett, the solicitor for Ramazan Aslan, owner of the fish bar, said his client was co-operating fully with the investigation. He said Mr Aslan's thoughts were with the families of those who are ill.
The other two cases have not been confirmed as being linked as yet.
He said: "He wants to help them as much as he can. Anyone in the catering industry in the same position would want to help the authorities to do their job."
Dr Judy Hart, consultant in communicable disease control at the National Public Health Service (NPHS) for Wales said along with Wrexham Council's environmental health officers they were investigating four cases of E.coli O157 in three families from the Wrexham area.
She said two people had been hospitalised and as a precautionary measure, a food premises was closed by the council on 30 July while investigations are carried out.
Dr Hart added: "The symptoms of E.coli O157 range from mild diarrhoea, stomach cramps and fever to bloody diarrhoea.
"Most people recover without complications, but the most severe cases can develop kidney failure.
"Anyone who is experiencing these symptoms should contact NHS Direct Wales for advice on 0845 4647."
She said people could become infected with E.coli O157 by eating contaminated food, through contact with farm animals or through contact with contaminated water.
"The infection can also pass from person to person and so it is important that anyone who is ill should observe strict personal hygiene to avoid spreading the infection," said Dr Hart.
"The incubation period for E.coli O157 can range from one to 14 days, but is characteristically three to four days. The length of the illness can vary but people with E.coli O157 are usually ill for up to two weeks."
She said investigations were ongoing and the NPHS and Wrexham Council would continue to monitor the situation closely.
